Journal:

Article Title: Tissue engineering of replacement skin: the crossroads of biomaterials, wound healing, embryonic development, stem cells and regeneration

doi: 10.1098/rsif.2006.0179

Figure Lengend Snippet: Examples of commercially created skin substitutes.

Article Snippet: Myskin CellTran , cultured autologous keratinocytes on a PVC polymer coated with a plasma-polymerized surface , Myskin with dermal fibroblasts under development , PVC encourages keratinocyte attachment and proliferation, providing a more stable delivery platform. Keratinocytes can be thawed for repeated application , up to 14 days required for cell expansion. Repeated application needed for good clinical outcome.

Techniques: Cell Culture, Membrane, Polymer, Clinical Proteomics, Migration

Journal: Nanoscale Advances

Article Title: Nanoparticles and bioorthogonal chemistry joining forces for improved biomedical applications

doi: 10.1039/d0na00873g

Figure Lengend Snippet: Biomedical applications based on the combined use of bioorthogonal chemistry and nanoparticles. (a), (b) and (c) denote the type of bioorthogonal reaction as depicted in Fig. 1 . The examples discussed more in detail in this review are marked with an asterisk

Article Snippet: , , Biomimetic (T cell membrane-coated) polymeric nanoparticles , In vivo enhanced photothermal therapy a , , , 2019.

Techniques: Ligation, Polymer, In Vivo, In Vitro, Bacteria, Imaging, Membrane, In Vivo Imaging, Infection, Liposomes, Positron Emission Tomography, Cell Tracking Assay, Diagnostic Assay, Amplification, Biomarker Discovery, Activation Assay, Drug discovery, Micro-PAT, Fluorescence, Transfection

Journal: Nanoscale Advances

Article Title: Nanoparticles and bioorthogonal chemistry joining forces for improved biomedical applications

doi: 10.1039/d0na00873g

Figure Lengend Snippet: Bioorthogonal labelling of integrin α5 membrane proteins using azide-modified antibodies and alkyne-HPGFNDs: (a) flow cytometry analysis of fluorescence signals from HFW cells preincubated with Azido-5αAb (red) and control cells (blue). (b) HFW cells labelled with Alexa Fluor 488-conjugated wheat germ agglutinin (i–iii) and 100 nm alkyne-HPGFNDs (iv–vi). White arrows indicate the cell migration route and blue arrows show the migration of integrin α5 on cells filopodia. Scale bars: 20 μm. Adapted with permission from ACS Appl. Mater. Interfaces, 11, Hsieh et al. , “Bioorthogonal Fluorescent Nanodiamonds for Continuous Long-Term Imaging and Tracking of Membrane Proteins”, 19774–19781. Copyright (2019) American Chemical Society.

Article Snippet: , , Biomimetic (T cell membrane-coated) polymeric nanoparticles , In vivo enhanced photothermal therapy a , , , 2019.

Techniques: Membrane, Modification, Flow Cytometry, Fluorescence, Control, Migration, Imaging

Journal: Nanoscale Advances

Article Title: Nanoparticles and bioorthogonal chemistry joining forces for improved biomedical applications

doi: 10.1039/d0na00873g

Figure Lengend Snippet: BCN-modified glycol chitosan nanoparticles for cell stem labelling and imaging. Top: Schematic structures (a). Bottom: NIRF (b), T 2 -weighted MRI (c) and micro CT (d) imaging of mice after transplantation of BCN–CNP-Cy5.5/IRON/GOLD labelled stem cells with and without Ac 4 ManNAz pre-treatment. Adapted from Biomaterials, 139, S. Lee et al. , “ In vivo stem cell tracking with imageable nanoparticles that bind bioorthogonal chemical receptors on the stem cell surface”, 12–29, Copyright (2017), with permission from Elsevier.

Article Snippet: , , Biomimetic (T cell membrane-coated) polymeric nanoparticles , In vivo enhanced photothermal therapy a , , , 2019.

Techniques: Modification, Imaging, Micro-CT, Transplantation Assay, In Vivo, Cell Tracking Assay

Journal: Nanoscale Advances

Article Title: Nanoparticles and bioorthogonal chemistry joining forces for improved biomedical applications

doi: 10.1039/d0na00873g

Figure Lengend Snippet: Pretargeting approach using SPAAC click chemistry between DBCO-MSNs and azide-labelled radiotracers (a) and PET-CT imaging of mice bearing U87 tumours (b and c). Mice lacking the pretargeting treatment (control, b) showed rapid renal excretion of the radiotracer. In mice treated with DBCO-MSNs (c) a persistent PET signal in the tumour site could be observed up two 120 min post-injection of the tracer. White arrows indicate kidneys (K) and tumour (T). Adapted from Angew. Chem., Int. Ed., 52, S. Lee et al. , “Mesoporous silica nanoparticle pretargeting for PET imaging based on a rapid bioorthogonal reaction in a living body”, 10549–10552, Copyright (2013), with permission from John Wiley & Sons Ltd.

Article Snippet: , , Biomimetic (T cell membrane-coated) polymeric nanoparticles , In vivo enhanced photothermal therapy a , , , 2019.

Techniques: Positron Emission Tomography-Computed Tomography, Imaging, Control, Injection

Journal: Nanoscale Advances

Article Title: Nanoparticles and bioorthogonal chemistry joining forces for improved biomedical applications

doi: 10.1039/d0na00873g

Figure Lengend Snippet: Overview of the BOND strategy. Left: Covalent coupling of TCO-antibodies and Tz-MFNPs. Right: Application of the BOND strategy for one-step (direct) and two-step (bioorthogonal amplification) targeting of MFNPs to cells. Reprinted by permission from Springer Nature, Nature Nanotechnology, “Bioorthogonal chemistry amplifies nanoparticle binding and enhances the sensitivity of cell detection”, J. B. Haun, N. K. Devaraj, S. A. Hilderbrand, H. Lee and R. Weissleder, Copyright (2010).

Article Snippet: , , Biomimetic (T cell membrane-coated) polymeric nanoparticles , In vivo enhanced photothermal therapy a , , , 2019.

Techniques: Amplification, Binding Assay

Journal: Nanoscale Advances

Article Title: Nanoparticles and bioorthogonal chemistry joining forces for improved biomedical applications

doi: 10.1039/d0na00873g

Figure Lengend Snippet: (a) Scheme of the labelling, amplification and cleavage process. (b) Fluorescent images from the labelling step and subsequent amplification steps of cells presenting HER2, and cells after DTT cleavage. (c) NMR signal analysis for HER2-targeted cells and cellular detection threshold for the different cleavage methods (labelling, AMP1-C, and AMP2-C). (d) Human clinical samples from pancreatic cancer assessed with AMP1 and AMP1-C strategies for different biomarkers (EGFR, EpCAM, HER2, MUC1). Adapted with permission from ACS Nano, 6, Peterson et al. , “Orthogonal amplification of nanoparticles for improved diagnostic sensing”, 3506–3513. Copyright (2012) American Chemical Society.

Article Snippet: , , Biomimetic (T cell membrane-coated) polymeric nanoparticles , In vivo enhanced photothermal therapy a , , , 2019.

Techniques: Amplification, Diagnostic Assay

Journal: Nanoscale Advances

Article Title: Nanoparticles and bioorthogonal chemistry joining forces for improved biomedical applications

doi: 10.1039/d0na00873g

Figure Lengend Snippet: μNMR analysis of glioblastoma-derived microvesicles and treatment response monitoring. (a) Scanning electron microscopy (SEM) image of a primary human glioblastoma cell, releasing abundant microvesicles. (b) High-magnification SEM image revealing saucer-shape exosomes on the surface of the cells. (c) Transmission electron microscopy (TEM) images of MNP-targeted exosomes. (d) BOND-2 strategy for the amplified binding of MNPs to protein biomarkers on the surface of microvesicles. (e) Design of the microfluidic system used for on-chip detection of circulating microvesicles. (f) Comparison of microvesicle detection sensitivity of different techniques, showing superior performance of μNMR when compared to western blotting (WB), flow cytometry (FC), enzyme-linked immunosorbent assay (ELISA) and nanoparticle tracking analysis (NTA). (g and h) Clinical trial results. Plots showing TPI values (g) and their corresponding η MV values (h) from the same patients before and after combined TMZ and radiation treatment. Reprinted by permission from Springer Nature, Nature Medicine, “Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy”, H. Shao, J. Chung, L. Balaj, A. Charest, D. D. Bigner, B. S. Carter, F. H. Hochberg, X. O. Breakefield, R. Weissleder and H. Lee, Copyright (2012).

Article Snippet: , , Biomimetic (T cell membrane-coated) polymeric nanoparticles , In vivo enhanced photothermal therapy a , , , 2019.

Techniques: Derivative Assay, Electron Microscopy, Transmission Assay, Amplification, Binding Assay, Comparison, Western Blot, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Nanoscale Advances

Article Title: Nanoparticles and bioorthogonal chemistry joining forces for improved biomedical applications

doi: 10.1039/d0na00873g

Figure Lengend Snippet: (a) Dose-dependent generation of azide groups on the surface of A549 cells. (b) Fluorescence intensity enhancement due to the multivalent effect of nanoparticles at the same concentration of azide precursor. Red: DBCO. Blue: DAPI (cell nucleus stain) (c) ex vivo NIRF analysis of tumour tissue labelling with DBCO-lipo and DBCO-Cy5. Reprinted from Angew. Chem., Int. Ed., 51, H. Koo et al. , “Bioorthogonal copper-free click chemistry in vivo for tumor-targeted delivery of nanoparticles”, 11836–11840, Copyright (2012), with permission from John Wiley & Sons Ltd.

Article Snippet: , , Biomimetic (T cell membrane-coated) polymeric nanoparticles , In vivo enhanced photothermal therapy a , , , 2019.

Techniques: Fluorescence, Concentration Assay, Staining, Ex Vivo, In Vivo

Journal: Nanoscale Advances

Article Title: Nanoparticles and bioorthogonal chemistry joining forces for improved biomedical applications

doi: 10.1039/d0na00873g

Figure Lengend Snippet: a) and (b) Illustration of the concept of administration of synergistic prodrug linked to TCO and their controlled, time-staggered activation in response to a tetrazine stimulus in vivo and in vitro . (c) Therapeutic response of BT-20 TNBC cells to the controlled activation of prodrugs loaded on MNPs. Left: Schematic representation of the [PAC1–TCO fast –MNP–TCO slow –DOX, MNP–D s P f ] construct, which showed the highest therapeutic efficacy. Right: Cell viability following administration of different constructs: MNP, MNP–D f , MNP–P f , MNP–D f P f and MNP–D s P f alone failed to induce cellular death, indicating inactivation of the drugs when immobilized on the MNP surface, while tetrazine administration activates the prodrugs and induces cellular death, with the greatest effect for MNP–D s P f (Note: s and f stand for slow and fast, respectively). Republished with permission of The Royal Society of Chemistry, from “Single-trigger dual-responsive nanoparticles for controllable and sequential prodrug activation”, N. M. Robertson, Y. Yang, I. Khan, V. E. LaMantia, M. Royzen and M. V. Yigit, Nanoscale, 2017, 9, 10020–10030; permission conveyed through Copyright Clearance Center, Inc.

Article Snippet: , , Biomimetic (T cell membrane-coated) polymeric nanoparticles , In vivo enhanced photothermal therapy a , , , 2019.

Techniques: Activation Assay, In Vivo, In Vitro, Clinical Proteomics, Construct, Drug discovery